Triple Compartment
Protein Trafficing URGENT Question?
You build a cDNA for a protein fusion triple hybrid. Ensure that sensitive protein VSVG temperature is located on the 3 'end followed by the gene for beta subunit of ATP synthase, followed by the GFP gene. State the reason traffiing that would be observed in the pH and osmotic balance of cells at 25 ° C by selecting the appropriate compartments in the list below. A Rough endoplasmic reticulum-Golgi Complex B-C-D-Cytosol mitochondrial matrix Late Endosomes E-F-G-Lysosome Plasma Membrane H-matrix I-J-inner mitochondrial membrane chloroplast K-stroma COPII vesicles Thus, in addition to the order, I do not understand the meaning of the B subunit of ATP-sythase. What to do? What would happen if it was located the 3 'end of the cDNA? What would happen if the gene for GFP was at the end 3 '? of the cDNA?
Good luck with that. I do not know much but I remember bio and chloroplasts in plant cells, so wat does that have to do with the protein?
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